Western blot /gel electrophoresis/isolate protein

Please complete the following test:

-Western blot  for  G6PD deficiency

-Gel electrophoresis

-Isolate Protein

USE THE FOLLOWING SEQUENCE :

Forward Primer: 5’- AACCGCATCATCGTGGAGAAG-3′ 

Reverse Primer: 5’-AGCCGGTCAGAGCTCTGCAGG-3′ 

Restriction enzyme- Glam11 

5’-AACGTT -3′ 

3’-TTGCAA-5′ 

5’-ACCGATCGACGTAA | ACGTTCGCAGATCTA | ACGTTCGCAGT-3′ 

3’-TGGCTAGCTGCATTTGCA | AGCGTCTAGATTGCA | AGCGTCA-5′ 

I NEED THIS DONE IN 6 (SIX ) HOURS

CLEAR PICTURES AND INFORMATION NEEDED

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