Sci 250 week 1 staining lab and quiz
SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each)
Check or underline the correct answer
Section: Multiple Choice
1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
- Gram stain
- Schaeffer-Fulton stain
- Acid-fast stain
- Lipid stain
- Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
- Capsular stain
- Gram stain
- Spore stain
- Negative stain
- Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.
- purple, safranin
- purple, crystal violet
- red, safranin
- red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure?
- A mordant causes the crystal violet to run.
- A mordant fixes the bacteria to the slide.
- A mordant keeps dye attached to an object.
- All of the above
5. What is the order of reagents used in the Gram stain?
- crystal violet, iodine, safranin, alcohol
- alcohol, crystal violet, iodine, safranin
- iodine, crystal violet, safranin, alcohol
- crystal violet, iodine, alcohol, safranin
- crystal violet, safranin, alcohol, iodine
6. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________
- spore staining.
- scanning electron microscopy.
- Gram staining
- Negative stain
Section: True or False
7. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
8. Older, pure cultures of Gram-positive organisms can appear red when Gram stained.
Section: Matching
9. Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
10. A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stai
- Cationic dye (also called basic dye)
11. Technique of staining the background around a specimen, leaving the specimen clear and unstained.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
12. A differential stain used to make endospores easier to visualize.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
13. A single dye used to reveal basic cell shapes and arrangements.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
14. A differential stain for organisms that are not decolorized in alcohol, such as the bacteria that cause Hansen’s Disease (leprosy) and tuberculosis.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
15. An ionic compound, used for staining bacteria, in which the positive ion imparts the color.
- Differential stain
- Flagellar stain
- Gram stain
- Negative stain
- Schaeffer-Fulton spore stain
- Simple stain
- Ziehl-Neelsen acid-fast stain
- Cationic dye (also called basic dye)
